Friday, 9 September 2011

MRSA st130 ( cc130 ) found in humans in Germany


Another strain of MRSA, already found in British livestock and humans, is reported in humans in Germany.

A few extracts from the report which drew the eye, follow.

Really it confirms what we already know: antibiotic resistant strains are moving from livestock into humans, with the facts and the risks massively under-reported in Britain.

British veterinarians do not want the risks or their role reported, and hijack the mass media with platitudes.

The problems do not generally get reported in Britain. After many years of cover-up by Defra, the British agriculture ministry, the real situation is bound to emerge.

It will be the veterinary organisations who are sitting on the pressure valve. British veterinary science is still in serious trouble and the situation is rapidly deteriorating.

Full report here

Rare Occurrence of Methicillin-Resistant Staphylococcus aureus CC130 with a Novel mecA Homologue in Humans in Germany

Christiane Cuny, Franziska Layer, Birgit Strommenger,Wolfgang Witte*
Wernigerode Branch, Robert Koch Institute, Wernigerode, Germany

Abstract Top

MRSA CC130 containing the mecA homologue mecALGA251 were reported from the UK and from Denmark so far from cattle and humans. Here we report on 11 MRSA CC130 among a sample of 12691 isolates of human origin collected from January 2006 until June 2011. MRSA CC130 grew insufficiently on chromogernic agar plates for detection of MRSA; the agglutination test for presence of PBP2a was negative. We designed primers for specific detection of mecALGA251 as well as for concomitant detection of both, mecLGA251 and mecA. As already described, the isolates exhibited spa-types t843, t1736, and t1773. The ccrA homologue indicated the presence SCCmecXI. When subjected to further characterization by means of a commercially available microarray the isolates were negative for sak chp, and scn, and as expected positive for hla, untruncated hlb, and hld. They furthermore contained edinB, aur, slpA, slpB, slpE. From genes coding for surface and cell wall associated products the ica-operon, cap8, clfA, clfF,ebpS, fnbA, fnbB, sdrC were detected but not cna. The isolates were negative for enterotoxin genes and tst, as well as for eta, and etb; agr-type was III.

 Earlier studies based on phenotypic characterization suggested that S. aureus from cattle and from humans are unrelated [7]. This was confirmed by molecular typing for clonal lineages ST97 and ST705 of bovine origin, which seem to be pandemic [8], [9]. Mastitis associated lineages ST151 and ST133 seem to be frequent in the United Kingdom (UK) and in Denmark [10], [11]. However the majority of isolates from these lineages remained methicillin susceptible…

 Another example for a clonal lineage with no restricted host specificity is S. aureus ST130 which represents a smaller fraction among S. aureus from mastitis in cattle in UK and more recently also MRSA ST130 from humans have been described [17], [18]. Interestingly these MRSA contain the mecA homologue mecLGA251 which is not detected by PCR established for detection of mecA. When human isolates exhibiting MRSA phenotype but negative PCR for mecA were tested, mecALGA251 was also detected among MRSA from humans in England and in Denmark. Here we report about the emergence of MRSA ST130 in infections in humans in Germany at low frequencies…

…Although MRSA ST130 have not been reported from cattle in Germany so far an animal origin seems likely, this is also supported by its isolation from a nasal swab of a veterinarian in Bavaria. As reported from Denmark and from the UK MRSA ST130 is obviously able to colonize and to cause infections in both, cattle and humans